RESUMO
The iconic phrase "a shot heard 'round the world" signifies an exceptional event. Seurat's masterpiece La Grande Jatte, painted with many thousand dots of color, came as a shot to the art world-a shot fired by the imagination of the artist and inspired by the color theories of a scientist.
Assuntos
Distinções e Prêmios , Percepção de Cores , Cor , Criatividade , Ilusões Ópticas , Pinturas/história , Química , Corantes/química , França , História do Século XIX , História do Século XX , Humanos , PinturaRESUMO
The neurotransmitter dopamine (DA) controls multiple behaviors and is perturbed in several major brain diseases. DA is released from large populations of specialized structures called axon varicosities. Determining the DA release mechanisms at such varicosities is essential for a detailed understanding of DA biology and pathobiology but has been limited by the low spatial resolution of DA detection methods. We used a near-infrared fluorescent DA nanosensor paint, adsorbed nanosensors detecting release of dopamine (AndromeDA), to detect DA secretion from cultured murine dopaminergic neurons with high spatial and temporal resolution. We found that AndromeDA detects discrete DA release events and extracellular DA diffusion and observed that DA release varies across varicosities. To systematically detect DA release hotspots, we developed a machine learningbased analysis tool. AndromeDA permitted the simultaneous visualization of DA release for up to 100 dopaminergic varicosities, showing that DA release hotspots are heterogeneous and occur at only â¼17% of all varicosities, indicating that many varicosities are functionally silent. Using AndromeDA, we determined that DA release requires Munc13-type vesicle priming proteins, validating the utility of AndromeDA as a tool to study the molecular and cellular mechanism of DA secretion.
Assuntos
Axônios , Dopamina , Neurônios Dopaminérgicos , Nanoestruturas , Neurotransmissores , Imagem Óptica , Animais , Axônios/metabolismo , Encéfalo/metabolismo , Dopamina/análise , Dopamina/metabolismo , Neurônios Dopaminérgicos/metabolismo , Corantes Fluorescentes/química , Camundongos , Neurotransmissores/análise , Neurotransmissores/metabolismo , Imagem Óptica/métodos , Pintura , Espectroscopia de Luz Próxima ao Infravermelho/métodosRESUMO
There is paucity of information about DNA methylation dynamics in immune cells. Roy et al. mapped the DNA methylation status of several thousand differentially methylated CpGs in human immune cells. They reported that the extent of cell type-specific hypermethylation is intriguingly most prevalent in adaptive immune cells rather than innate cells.
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Metilação de DNA , Fatores de Transcrição , Ilhas de CpG , Epigênese Genética , Humanos , PinturaRESUMO
For the control of biofouling, some paints based on compounds that are toxic to marine organisms have been used. There is an intensive search for biodegradable solutions that are friendly to non-target organisms. Bacteria have been shown to be a source of compounds with antifouling potential. In this work, the antifouling activity of a strain of Staphylococcus aureus was evaluated. Extracts activity against biofilm-forming bacteria and the toxicity against Artemia franciscana were evaluated. The extracts were incorporated in a hard gel and a paint matrix, and they were exposed to the sea. In both the laboratory and field, we found that the compounds produced by S. aureus have antifouling activity. The non-toxicity of the tested extracts against Artemia franciscana nauplii suggests that the extracts obtained from S. aureus could have a low ecological impact over non-target organisms. Significant differences were found in the percentage of organisms cover in hard gels with extracts and control. After 90 days, important differences were also observed between the percentage of organisms cover of the paints that contained extracts and the control. Dichloromethane extract is the most effective for the inhibition or delay of the settlement of organisms For this reason, they could be used in matrices with different applications, such as in the shipping industry, aquaculture, or any other in which biofouling is a cause of inconvenience.
Assuntos
Incrustação Biológica , Incrustação Biológica/prevenção & controle , Staphylococcus aureus , Biofilmes , PinturaRESUMO
Ozone and its oxidation products result in negative health effects when inhaled. Despite painted surfaces being the most abundant surface in indoor spaces, surface loss remains one of the largest uncertainties in the indoor ozone budget. Here, ozone uptake coefficients (γO3) on painted surfaces were measured in a flow-through reactor where 79% of the inner surfaces were removable painted glass sheets. Flat white paint initially had a high uptake coefficient (8.3 × 10-6) at 20% RH which plateaued to 1.1 × 10-6 as the paint aged in an indoor office over weeks. Increasing the RH from 0 to 75% increased γO3 by a factor of 3.0, and exposure to 134 ppb of α-terpineol for 1 h increased γO3 by a factor of 1.6 at 20% RH. RH also increases α-terpineol partitioning to paint, further increasing ozone loss, but the type of paint (flat, eggshell, satin, semigloss) had no significant effect. A kinetic multilayer model captures the dependence of γO3 on RH and the presence of α-terpineol, indicating the reacto-diffusive depth for O3 is 1 to 2 µm. Given the similarity of the kinetics on aged surfaces across many paint types and the sustained reactivity during aging, these results suggest a mechanism for catalytic loss.
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Ozônio , Pintura , Ozônio/química , Umidade , Compostos Orgânicos Voláteis , Poluição do Ar em Ambientes FechadosRESUMO
Despite the extensive global consumption of architectural paint, the toxicological effects of aged exterior paint particles on terrestrial biota remain largely uncharacterized. Herein, we assessed the toxic effect of aged paint particles on soil environments using the nematode Caenorhabditis elegans (C. elegans) as a test organism. Various types of paint particles were generated by fragmentation and sequential sieving (500-1000, 250-500, 100-250, 50-100, 20-50 µm) of paint coatings collected from two old residential areas. The paint particles exerted different levels of toxicity, as indicated by a reduction in the number of C. elegans offspring, depending on their size, color, and layer structure. These physical characteristics were found to be closely associated with the chemical heterogeneity of additives present in the paint particles. Since the paint particle sizes were larger than what C. elegans typically consume, we attributed the toxicity to leachable additives present in the paint particles. To assess the toxicity of these leachable additives, we performed sequential washings of the paint particles with distilled water and ethanol. Ethanol washing of the paint particles significantly reduced the soil toxicity of the hydrophobic additives, indicating their potential environmental risk. Liquid chromatography-mass spectrometry analysis of the ethanol leachate revealed the presence of alkyl amines, which exhibited a high correlation with the toxicity of the paint particles. Further toxicity testing using an alkyl amine standard demonstrated that a paint particle concentration of 1.2% in soil could significantly reduce the number of C. elegans offspring. Our findings provide insights into the potential hazards posed by aged paint particles and their leachable additives in the terrestrial environment.
Assuntos
Caenorhabditis elegans , Solo , Animais , Solo/química , Ecossistema , Pintura , Etanol/farmacologiaRESUMO
BACKGROUND: Due to an increasing occupational usage of isothiazolinone (IT)-containing preservatives, and their potential to cause skin sensitization and allergic contact dermatitis, that is, chronic disease, there is a need for more knowledge on how highly exposed workers are affected. OBJECTIVES: The overall objective was to explore dermatological symptoms of potentially long-lasting or chronic character in Swedish painters. METHODS: Building painters from western and southern Sweden were initially invited to perform a questionnaire on occurrence of skin symptoms. Participants with affirmative responses, and the right inclusion criteria, were further invited to patch testing with four different ITs: benzisothiazolinone (BIT), methylisothiazolinone, methylchloroisothiazolinone and octylisothiazolinone. RESULTS: There was a tendency towards higher occurrence of positive patch test reactions among the painters compared with occupationally unexposed registry patients; however, not statistically significant differences. BIT was the substance most frequently causing positive test results in both groups. The occurrence of adult-onset eczema was higher in painters than in the control group of electricians, and just shy of statistical significance concerning any of several skin locations (face/legs/arms/hands). CONCLUSION: Building painters present with positive patch test reactions to common paint preservatives (ITs), and they report adult-onset eczema more often than do less occupationally exposed groups.
Assuntos
Dermatite Alérgica de Contato , Dermatite Ocupacional , Eczema , Exposição Ocupacional , Pintura , Testes do Emplastro , Conservantes Farmacêuticos , Tiazóis , Humanos , Tiazóis/efeitos adversos , Suécia/epidemiologia , Dermatite Alérgica de Contato/etiologia , Dermatite Alérgica de Contato/diagnóstico , Dermatite Ocupacional/etiologia , Dermatite Ocupacional/diagnóstico , Dermatite Ocupacional/epidemiologia , Masculino , Adulto , Pessoa de Meia-Idade , Conservantes Farmacêuticos/efeitos adversos , Exposição Ocupacional/efeitos adversos , Eczema/induzido quimicamente , Eczema/epidemiologia , Feminino , Pintura/efeitos adversos , Autorrelato , Inquéritos e QuestionáriosRESUMO
A simple and environmentally friendly method was developed for smart and efficient waterborne polyurethane (PUR) paint. Sugarcane bagasse was recycled into reduced graphene oxide nanosheets (rGONSs). Both lanthanide-doped aluminate nanoparticles (LAN; photoluminescent agent, 7-9 nm) and rGONSs (reinforcement agent) were integrated into a waterborne polyurethane to produce a novel photoluminescent, hydrophobic, and anticorrosive nanocomposite coating. Using ferrocene-based oxidation under masked circumstances, graphene oxide nanosheets were produced from sugarcane bagasse. The oxidized semicarbazide (SCB) nanostructures were integrated into polyurethane coatings as a drying, anticorrosion, and crosslinking agent. Polyurethane coatings with varying amounts of phosphor pigment were prepared and subsequently applied to mild steel. The produced paints (LAN/rGONSs@PUR) were tested for their hydrophobicity, hardness, and scratch resistance. Commission Internationale de l'éclairage (CIE) Laboratory parameters and photoluminescence analysis established the opacity and colourimetric properties of the nanocomposite coatings. When excited at 365 nm, the luminescent transparent paints emitted a strong greenish light at 517 nm. The anticorrosion characteristics of the coated steel were investigated. The phosphor-containing (11% w/w) polyurethane coatings displayed the most pronounced anticorrosion capability and long-persistent luminosity. The prepared waterborne polyurethane paints were very photostable and durable.
Assuntos
Grafite , Interações Hidrofóbicas e Hidrofílicas , Nanocompostos , Pintura , Poliuretanos , Poliuretanos/química , Grafite/química , Nanocompostos/química , Luminescência , Corrosão , Química VerdeRESUMO
Workers in painting companies may be unaware that the paints they handle have adverse health effects. The present study explored the factors associated with workers' perception of paints as "dangerous and harmful". A questionnaire survey and interviews were conducted with workers engaged in painting operations in small-sized painting companies (fewer than 50 employees) in northern Kyushu and Yamaguchi prefectures. Safety and health education methods making workers perceive paint as "dangerous and harmful" were clarified. Fisher's exact test and logistic regression analysis were used for the survey and hierarchical cluster analysis for the interviews. The factor "Hazard prediction activity (KY Activity)", gender, and job position were identified as factors that make workers perceive paint as "dangerous and harmful". Based on the interview results, "KY Activity" was a keyword and a standardized safety and health activity. Before work, workers gather in a small group to discuss the dangers and hazards of paint, share information, establish action goals, and confirm safety measures. The questionnaire identified "KY Activity" as a factor influencing the workers' perception of paint as "dangerous and harmful". "KY Activity," also extracted from the interviews, is an effective health and safety education method for teaching workers in small-sized painting companies that paint is "dangerous and harmful".
Assuntos
Saúde Ocupacional , Pintura , Humanos , Masculino , Feminino , Inquéritos e Questionários , Saúde Ocupacional/educação , Adulto , Educação em Saúde/métodos , Pessoa de Meia-IdadeRESUMO
Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) continues to threaten lives by evolving into new variants with greater transmissibility. Although lateral flow assays (LFAs) are widely used to self-test for coronavirus disease 2019 (COVID-19), these tests suffer from low sensitivity leading to a high rate of false negative results. In this work, a multiplexed lateral flow assay is reported for the detection of SARS-CoV-2 and influenza A and B viruses in human saliva with a built-in chemical amplification of the colorimetric signal for enhanced sensitivity. To automate the amplification process, the paper-based device is integrated with an imprinted flow controller, which coordinates the routing of different reagents and ensures their sequential and timely delivery to run an optimal amplification reaction. Using the assay, SARS-CoV-2 and influenza A and B viruses can be detected with ≈25x higher sensitivity than commercial LFAs, and the device can detect SARS-CoV-2-positive patient saliva samples missed by commercial LFAs. The technology provides an effective and practical solution to enhance the performance of conventional LFAs and will enable sensitive self-testing to prevent virus transmission and future outbreaks of new variants.
Assuntos
COVID-19 , Herpesvirus Cercopitecino 1 , Influenza Humana , Humanos , SARS-CoV-2 , COVID-19/diagnóstico , Influenza Humana/diagnóstico , Pintura , Sensibilidade e EspecificidadeRESUMO
Although the global ban on leaded gasoline has markedly reduced lead poisoning, many other environmental sources of lead exposure, such as paint, pipes, mines, and recycling sites remain. Existing methods to identify these sources are either costly or unreliable. We report here a new, sensitive, and inexpensive lead detection method that relies on the formation of a perovskite semiconductor. The method only requires spraying the material of interest with methylammonium bromide and observing whether photoluminesence occurs under UV light to indicate the presence of lead. The method detects as little as 1.0 ng/mm2 of lead by the naked eye and 50 pg/mm2 using a digital photo camera. We exposed more than 50 different materials to our reagent and found no false negatives or false positives. The method readily detects lead in soil, paint, glazing, cables, glass, plastics, and dust and could be widely used for testing the environment and preventing lead poisoning.
Assuntos
Exposição Ambiental , Intoxicação por Chumbo , Humanos , Compostos de Cálcio , Óxidos , Intoxicação por Chumbo/prevenção & controle , Pintura , PoeiraRESUMO
Face paints used by opera performers have been shown to contain high levels of heavy metals. However, whether frequent exposure, via dermal contact and inadvertent oral ingestion, results in occupational diseases is unknown, as is the potential exacerbation of toxicity by high-intensity irradiation from stage lights. In this study, we examined the release of Cr, Cu, Pb, and Zn from 40 face paints and the consequent health risks posed by different practical scenarios involving their use. The results showed that the in vitro bioaccessibility (IVBA) of Cr, Cu, Pb, and Zn in the tested products was, on average, 7.0, 5.5, 19.9, and 7.9% through oral ingestion and 1.1, 2.2, 1.6, and 1.2% through dermal contact, respectively. Stage light irradiation significantly increased the IVBA associated with dermal contact, to the average of 4.8, 34.9, 5.7, and 1.9% for Cr, Cu, Pb, and Zn, respectively. The increase was mainly due to the light-induced generation of reactive oxygen species, particularly hydroxyl free radicals. The vitality and transcriptional response of 3D skin models as well as a quantitative risk assessment of skin sensitization indicated that dermal contact with face paints may induce predictable skin damage and potentially other skin diseases. Long-term exposure to face paints on stage may also pose a non-carcinogenic health risk. The demonstrated health risks to opera performers of face paint exposure should lead to strict regulations regarding the content of theatrical face paints.
Assuntos
Metais Pesados , Poluentes do Solo , Espécies Reativas de Oxigênio , Monitoramento Ambiental , Chumbo , Pintura , Medição de Risco/métodos , ChinaRESUMO
Housing-based lead paint dust is the most common source of lead exposure for US-born children. Although year of housing construction is a critical indicator of the lead hazard to US children, not all housing of the same age poses the same risk to children. Additional information about housing condition is required to differentiate the housing-based lead risk at the parcel level. This study aimed to identify and assess a method for gathering and using observations of exterior housing conditions to identify active housing-based lead hazards at the parcel level. We used a dataset of pediatric blood lead observations (sample years 2000-2013, ages 6-72 months, n = 6,589) to assess associations between observations of exterior housing conditions and housing-based lead risk. We used graphical and Lasso regression methods to estimate the likelihood of an elevated blood lead observation (≥3.5 µg/dL). Our methods estimate a monotonic increase in the likelihood of an elevated blood lead observation as housing conditions deteriorate with the largest changes associated with homes in the greatest disrepair. Additionally we estimate that age of home construction works in consort with housing conditions to amplify risks among those houses built before 1952. Our analysis indicates that a survey of external housing conditions can be used in combination with age of housing in the identification process, at the parcel level, of homes that pose a housing-based lead hazard to children.
Assuntos
Intoxicação por Chumbo , Chumbo , Criança , Humanos , Lactente , Pré-Escolar , Chumbo/análise , Intoxicação por Chumbo/epidemiologia , Intoxicação por Chumbo/etiologia , Qualidade Habitacional , Exposição Ambiental/análise , Habitação , Poeira/análise , Pintura/análiseRESUMO
A laboratory test with a flow-through system was designed and its applicability to testing antifouling paints of varying efficacies was investigated. Six different formulations of antifouling paints were prepared to have increased contents (0 to 40 wt%) of Cu2O. The test plates were initially aged for 45 days by rotating them at a speed of 10 knots inside a cylinder drum. A bioassay was then conducted using the alga, Ectocarpus sp., as the test species. The new bioassay for screening antifouling paints under a flow-through system was successfully established using algae affixed on substrata. The correlation between the mean values of CIELAB parameters (L*, a* and b*), the total colour difference (ΔE*), and the cell survival rate of the algae was investigated. The paint performance estimated from the bioassay was confirmed using correlation patterns between colour parameters and the algal cell survival rate.
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Incrustação Biológica , Incrustação Biológica/prevenção & controle , Biofilmes , Pintura , BioensaioRESUMO
Ships in service feature surfaces that exhibit biofouling, which alters the hydrodynamics of the vessels, thus affecting their normal displacement and significantly increasing their fuel consumption. The application of three types of ceramic coatings as ecological, effective and durable alternatives to commercial silicone-based marine coatings is investigated in this study. Three different ceramic glazes and two control commercial paints are analysed in an actual environment during 20 months of exposure to simulate the navigation conditions such that growth and roughness data can be obtained and then applied to computational fluid dynamics (CFD) software using an open-source Reynolds-averaged Navier-Stokes solver. The CFD results are validated under smooth hull conditions with a full-scale Kriso Container Ship (KCS) model and with different levels of hull roughness. The developed approach shows that the drag in hulls coated with conventional paint is 19% greater than that in hulls with ceramic coating.
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Biofilmes , Incrustação Biológica , Navios , Incrustação Biológica/prevenção & controle , Hidrodinâmica , PinturaRESUMO
High-pressure paint injection injury to the orbit is a rare type of orbital trauma. We present an unfortunate case of high-pressure paint injury to the right orbit of a young patient. High-pressure injection injury presents with a unique injury mechanism and resultant deep tissue damage. The superficial appearance of the entry site injury is deceiving; therefore, a thorough evaluation is necessary. Debridement is usually required if foreign body material is present. Antibiotics and steroids are commonly used in such cases.
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Traumatismos Oculares , Doenças Orbitárias , Humanos , Órbita/diagnóstico por imagem , Órbita/lesões , PinturaRESUMO
PURPOSE: To evaluate the effect of different varnishes on dentin permeability to erosive/abrasive challenges. METHODS: 60 superficial dentin discs were randomly allocated to six groups (n=10): Duraphat, PRG Barrier Coat, Clinpro XT, Profluorid, Fluor Protector S and untreated (control). Permeability tests were carried out: after acid conditioning, after treatment, and after abrasive/erosive challenges. The abrasive-erosive challenges were performed for 5 days (citric acid and in remineralizing saliva), as well as abrasion with toothpaste slurry (Colgate Total 12). Scanning electron microscopy (SEM) images were obtained of the dentin surfaces (5,000x) after the treatments, and after the erosive/abrasive challenges. RESULTS: Kruskal-Wallis and Dunn tests showed that all the varnishes promoted a decrease in dentin permeability directly after application, with Clinpro XT showing the highest percentage (69.52%) of dentin sealing (P= 0.0188). After the erosive/abrasive challenge, only PRG Barrier Coat varnish showed dentin sealing ability with reduced dentin permeability (42.16%) (P= 0.0014). However, none of the applied varnishes promoted a statistically significant difference in the percentage of dentin sealing, compared with the control group (P> 0.05). Clinpro XT showed a higher percentage of dentin sealing, while only PRG Barrier Coat showed reduced dentin permeability after the erosive/abrasive challenge. CLINICAL SIGNIFICANCE: Varnish containing S-PRG filler has the efficacy needed to be used as a coating material to reduce dentin permeability in situations of erosive/abrasive challenges.
Assuntos
Ácido Cítrico , Permeabilidade da Dentina , Pintura , Microscopia Eletrônica de Varredura , Saliva , Cremes Dentais , DentinaRESUMO
This study focused on the antifouling effect of copper oxide (Cu2O)- and zineb-based coatings against Cyanothece sp. ATCC 51142 by analysing photosynthetic activity using chlorophyll fluorescence. The photoautotrophically grown cyanobacterium was exposed to toxic coatings over a short-term period of 32 h. The study showed that Cyanothece cultures are particularly sensitive to biocides (i) released from antifouling paints and (ii) exhibited by contact with the coated surfaces. Changes in the maximum quantum yield of photosystem II (FV/FM) were observed within the first 12 h of exposure to the coatings. Partial recovery of FV/FM in Cyanothece was revealed 24 h post exposure to a copper- and zineb-free coating. In this research, we proposed an analysis of the evaluation of fluorescence data to study the initial response of cyanobacterial cells to copper- and non-copper-based antifouling coatings formulated with zineb. We evaluated the dynamics of coating toxicity by determining the characteristic time constants of changes in the FV/FM. Within the most toxic paints studied, those formulated with the highest concentration of Cu2O and zineb, the estimated time constants were 3.9 times lower compared to the copper- and zineb-free paint. The use of zineb in copper-based antifouling coatings enhanced the toxic effect of paints and contributed to a faster decline in photosystem II activity in Cyanothece cells. The analysis we proposed, along with the fluorescence screening results, may be useful in evaluating the initial antifouling dynamic action against photosynthetic aquacultures.
Assuntos
Incrustação Biológica , Cianobactérias , Desinfetantes , Fluorescência , Desinfetantes/análise , Incrustação Biológica/prevenção & controle , Complexo de Proteína do Fotossistema II , Navios , PinturaRESUMO
The present study experimentally quantified the pyrolysis behaviors of waste solvent-based automotive paint sludge (OAPS) and water-based automotive paint sludge (WAPS) at four different heating rates using thermogravimetric-Fourier transform infrared (TG-FTIR) spectrometry and pyrolysis-gas chromatography-mass (Py-GC/MS) spectrometry analyses. Flynn-Wall-Ozawa (FWO) and Kissinger-Akahira-Sunose (KAS) methods combined with the master-plots method were employed to investigate the pyrolysis kinetics and reaction mechanisms of waste automotive paint sludge. Three reaction stages and three reaction peaks in stage 2 were distinguished for both OAPS and WAPS degradation. The average activation energy (Ea) estimates for OAPS (FWO: 179.09 kJ/mol; KAS: 168.28 kJ/mol) were slightly higher than WAPS (FWO: 175.90 kJ/mol; KAS: 164.80 kJ/mol) according to FWO and KAS methods. The main pyrolysis reaction mechanisms of both OAPS and WAPS closely matched with the order-based model corresponding to 3rd and 2nd order random nucleation on an individual particle. The evolved gas species of CH4, CO2, phenols, NH3, H2O, and CO from OAPS and WAPS pyrolysis were identified by TG-FTIR. According to Py-GC/MS, hydrocarbons (47.2%) and O-components (42.7%) were relatively large after OAPS and WAPS pyrolysis, respectively. Melamine was the most abundant N-component product after pyrolysis of OAPS (5.8%) and WAPS (4.8%).
Assuntos
Pirólise , Esgotos , Cromatografia Gasosa-Espectrometria de Massas , Cinética , Espectroscopia de Infravermelho com Transformada de Fourier , Termogravimetria , Gases , PinturaRESUMO
The Natural History and Science Museum of the University of Porto houses a collection of 45 models of fungi in papier-mâché from the 19th-century, which were used at the university until 2015 as didactic models. For the first time, the materials and techniques used in the production of a Boletus edulis model were studied (vernacular name: cep, porcini). These sculptures, made to life-size scale, are painted in colors similar to those of the represented species (white, brown, and light brown). They are fixed to a rectangular base, which is painted black, and to which moss has been pasted. To fully characterize each color, at the molecular level, a multi-analytical approach was used, combining energy-dispersive x-ray fluorescence spectroscopy (micro-XRF) with fingerprinting techniques of Raman microscopy (microRaman and handheld Raman) spectroscopy and microFourier transform infrared spectroscopy (microFTIR). The papier-mâché was prepared with a groundwood paper to which kaolin and a quartz-based material have been added to reinforce the structure. Raman microscopy also identified carbon black in it, which is possibly responsible for its grey color. The white color was unequivocally identified as lithopone by microRaman. This white paint was prepared in a proteinaceous tempera, with calcium carbonate having been identified as filler (by microFTIR). In the brown color, iron was identified by microXRF, pointing to the use of ocher, which was not possible to identify by microRaman and microFTIR. Regarding the black rectangular base, the moss was fixed using a collagen-based glue. The binding medium in this black is possibly a mixture of drying oil and protein. Again, XRF detected iron as the main element, but it was not possible to acquire a Raman spectrum due to the high fluorescence of the binder/varnish. Others, such as the writing inks, will also be discussed. The colors identified are in line with the best materials available for use by artists of that time. This new knowledge is fundamental to informing the choice of the best conservation strategies for the preservation of these extraordinary models.