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Am J Dermatopathol ; 40(10): 727-735, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30188378

RESUMO

Hypopigmented interface T-cell dyscrasia (HITCD) is a distinct form of lymphoid dyscrasia that may progress to hypopigmented mycosis fungoides (HMF). We compared both diseases as regards their CD4/CD8 phenotype and expression of granzyme B and tumor necrosis factor-alpha (TNF-α) and how these are affected by narrow-band UVB (nb-UVB). The study included 11 patients with HITCD and 9 patients with HMF. They received nb-UVB thrice weekly until complete repigmentation or a maximum of 48 sessions. Pretreatment and posttreatment biopsies were stained using anti CD4, CD8, TNF-α, and granzyme B monoclonal antibodies. Epidermal lymphocytes were CD8 predominant in 54.5% and 66.7% of HITCD and HMF cases, respectively, whereas dermal lymphocytes were CD4 predominant in 63.6% and 66.7%, respectively. Significantly, more dermal infiltrate was encountered in HMF (P = 0.041). In both diseases, granzyme B was only expressed in the dermis, whereas TNF-α was expressed both in the epidermis and dermis. No difference existed as regards the number of sessions needed to achieve repigmentation or cumulative nb-UVB dose reached at end of study. (P > 0.05). Narrow-band UVB significantly reduced only the epidermal lymphocytes in both diseases (P ≤ 0.05) with their complete disappearance in 8 (72.7%) HITCD and 6 (66.7%) HMF cases. In both diseases, nb-UVB did not affect granzyme B or TNF-α expression (P > 0.05). In conclusion, both diseases share the same phenotype, with HITCD being a milder form of T-cell dysfunction. In both diseases, epidermal lymphocytes are mainly CD8-exhausted cells lacking cytotoxicity, whereas dermal cells are mostly reactive cells exerting antitumor cytotoxicity. Tumor necrosis factor-alpha mediates hypopigmentation in both diseases and prevents disease progression. Repigmentation after nb-UVB in both diseases occurs before and independently from disappearance of the dermal infiltrate.


Assuntos
Linfócitos T CD4-Positivos/patologia , Linfócitos T CD8-Positivos/patologia , Hipopigmentação/patologia , Transtornos Imunoproliferativos/patologia , Micose Fungoide/patologia , Neoplasias Cutâneas/patologia , Pigmentação da Pele , Pele/patologia , Adolescente , Adulto , Biópsia , Linfócitos T CD4-Positivos/química , Linfócitos T CD4-Positivos/efeitos da radiação , Linfócitos T CD8-Positivos/química , Linfócitos T CD8-Positivos/efeitos da radiação , Criança , Estudos Transversais , Feminino , Granzimas/análise , Humanos , Hipopigmentação/metabolismo , Hipopigmentação/radioterapia , Imuno-Histoquímica , Transtornos Imunoproliferativos/metabolismo , Transtornos Imunoproliferativos/radioterapia , Masculino , Pessoa de Meia-Idade , Micose Fungoide/química , Micose Fungoide/radioterapia , Fenótipo , Pele/química , Pele/efeitos da radiação , Neoplasias Cutâneas/química , Neoplasias Cutâneas/radioterapia , Pigmentação da Pele/efeitos da radiação , Resultado do Tratamento , Fator de Necrose Tumoral alfa/análise , Terapia Ultravioleta , Adulto Jovem
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