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Cisplatin-induced azoospermia and testicular damage ameliorated by adipose-derived mesenchymal stem cells
Ismail, Hamdy Y; Shaker, Nora A; Hussein, Shaymaa; Tohamy, Adel; Fathi, Mohamed; Rizk, Hamdy; Wally, Y. R.
Afiliação
  • Ismail, Hamdy Y; Cairo University. Faculty of Veterinary Medicine. Department of Anatomy and Embryology. Giza. EG
  • Shaker, Nora A; Cairo University. Faculty of Veterinary Medicine. Department of Anatomy and Embryology. Giza. EG
  • Hussein, Shaymaa; Cairo University. Faculty of Veterinary Medicine. Department of Cytology and Histology. Giza. EG
  • Tohamy, Adel; Cairo University. Faculty of Veterinary Medicine. Department of Toxicology & Forensic Medicine. Giza. EG
  • Fathi, Mohamed; Cairo University. Faculty of Veterinary Medicine. Department of Theriogenology. Giza. EG
  • Rizk, Hamdy; Cairo University. Faculty of Veterinary Medicine. Department of Anatomy and Embryology. Giza. EG
  • Wally, Y. R; Cairo University. Faculty of Veterinary Medicine. Department of Anatomy and Embryology. Giza. EG
Biol. Res ; 56: 2-2, 2023. ilus, tab, graf
Article em En | LILACS | ID: biblio-1420300
Biblioteca responsável: CL1.1
ABSTRACT

BACKGROUND:

The testes are highly susceptible to the adverse effects of chemotherapy and radiation at all stages of life. Exposure to these threats mainly occurs during cancer treatment and as an occupational hazard in radiation centers. The present study investigated the regenerative ability of adipose-derived mesenchymal stem cells (ADMSCs) against the adverse effects of cisplatin on the structure and function of the testes.

METHODS:

New Zealand white rabbits (N = 15) were divided into three groups of five a negative control group (no treatment), a cisplatin group (single dose of cisplatin into each testis followed three days later by a PBS injection), and a cisplatin + ADMSCs group (cisplatin injection followed three days later by an ADMSC injection). On day 45 post-treatment, serum testosterone levels were evaluated, and the testes and epididymis were collected for histology, oxidative stress examination, and epididymal sperm analysis.

RESULTS:

Cisplatin caused damage to the testicular tissue and decreased serum testosterone levels, epididymal sperm counts, and oxidants. An antioxidant imbalance was detected due to increasing malondialdehyde (MDA) and reduced glutathione (GSH) levels in testicular tissue. The ADMSC-treated group displayed a moderate epididymal sperm count, adequate antioxidant protection, suitable hormone levels, and enhanced testicular tissue morphology.

CONCLUSIONS:

ADMSCs treatment repaired damaged testicular tissue, enhanced biochemical parameters, and modified pathological changes caused by cisplatin.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Azoospermia Limite: Animals / Humans / Male Idioma: En Revista: Biol. Res Assunto da revista: BIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Egito

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Azoospermia Limite: Animals / Humans / Male Idioma: En Revista: Biol. Res Assunto da revista: BIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Egito