Remote ischemic preconditioning and tacrolimus in the fetal small bowel transplant in mice
Acta cir. bras
; 31(10): 675-679, Oct. 2016. graf
Article
em En
| LILACS
| ID: biblio-827651
Biblioteca responsável:
BR1.1
ABSTRACT
ABSTRACT PURPOSE:
To evaluate the effect of remote ischemic preconditioning (IPC-R) in the fetal small bowel transplantation model.METHODS:
Two groups were constituted The Isogenic transplant (ISO, C57BL/6 mice, n=24) and the allogenic transplant (ALO, BALB/c mice, n=24). In each group, the animals were distributed with and without IPC-R. It was obtained the following subgroups Tx, IPC-R, Fk, IPC-Fk, in both strains. Intestinal grafts were stained with hematoxylin and eosin and immunohistochemically.RESULTS:
The graft development evaluation in ISO group showed that IPC-R reduced the development compared with ISO-Tx (5.2±0.4 vs 9.0±0.8) and IPC-R-Fk increased the graft development compared with IPC-R (11.2±0.7 and 10.2±0.8). In ALO group, IPC-Fk increased the development compared with ALO-Tx and ALO with IPC-R (6.0±0.8, 9.0±1.2, 0.0±0.0, 0.5±0.3). The PCNA expression was increased in ISO group treated with Fk and IPC-R compared to other groups (12.2±0.8 vs Tx 8.8±0.9, IPC-R 8.0±0.4 and Fk 9.0±0.6). The graft rejection was lower in groups treated with IPC-R (-18%), Fk (-68%) or both (-61%) compared with ALO-Tx.CONCLUSION:
Remote ischemic preconditioning showed benefic effect even associate with Tacrolimus on the development and acute rejection of the fetal small bowel graft in the Isogenic and Allogenic transplants.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Transplante de Tecido Fetal
/
Tacrolimo
/
Precondicionamento Isquêmico
/
Imunossupressores
/
Intestino Delgado
Tipo de estudo:
Evaluation_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Brasil