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West Nile virus infection of primary mouse neuronal and neuroglial cells: the role of astrocytes in chronic infection
Diniz, José A. P.; Travassos da Rosa, Amelia P. A.; Guzman, Hilda; XU, Fangling; Xiao, Shu-Yuan; Popov, Vsevolod L.; Vasconcelos, Pedro F. C..
Afiliação
  • Diniz, José A. P.; Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Belém. Brasil
  • Travassos da Rosa, Amelia P. A.; University of Texas Medical Branch. Department of Pathology and Center for Biodefense and Emerging Infectious Diseases. Galveston. USA
  • Guzman, Hilda; University of Texas Medical Branch. Department of Pathology and Center for Biodefense and Emerging Infectious Diseases. Galveston. USA
  • XU, Fangling; University of Texas Medical Branch. Department of Pathology and Center for Biodefense and Emerging Infectious Diseases. Galveston. USA
  • Xiao, Shu-Yuan; University of Texas Medical Branch. Department of Pathology and Center for Biodefense and Emerging Infectious Diseases. Galveston. USA
  • Popov, Vsevolod L.; University of Texas Medical Branch. Department of Pathology and Center for Biodefense and Emerging Infectious Diseases. Galveston. USA
  • Vasconcelos, Pedro F. C.; Instituto Evandro Chagas. Departamento de Arbovirologia e Febres Hemorrágicas. Belém. Brasil
Am. j. trop. med. hyg ; 75(4): 691-696, 2006. ilus, tab, graf
Article em En | BVSDIP, FIOCRUZ | ID: dip-2018
Biblioteca responsável: BR275.1
Localização: BR275.1; PCIEC2006
ABSTRACT
Primary cultures of embryonic murine neurons and newborn mouse astrocytes were inoculated with West Nile virus (WNV) strain NY385-99 to compare the pathogenesis of WNV infection in these types of CNS cells. Two different outcomes were observed. WNV infection in the neurons was rapidly progressive and destructive; within 5 days, all of the neurons were destroyed through apoptosis. WNV infection in the astrocytes evolved more slowly and did not seem to be highly lethal to the cells. The infected astrocytes continued to produce infectious virus (10(4.6)-10(6.5) PFU/mL) for 114 days, in a permissive, persistent infection. During this period, WNV antigen could be shown in the cytoplasm of the infected astrocytes by immunocytochemical assay, transmission electron microscopy of ultrathin sections, and in the cell culture medium by complement fixation test. Our results with this in vitro experimental murine cell model indicate that astrocytes can develop chronic or persistent infection with WNV, suggesting that these cells may play a role in the maintenance of WNV in the CNS...(AU)
Assuntos
Texto completo: 1 Base de dados: BVSDIP / FIOCRUZ Assunto principal: Vírus do Nilo Ocidental / Astrócitos / Doença Crônica Limite: Animals Idioma: En Revista: Am. j. trop. med. hyg Ano de publicação: 2006 Tipo de documento: Article
Texto completo: 1 Base de dados: BVSDIP / FIOCRUZ Assunto principal: Vírus do Nilo Ocidental / Astrócitos / Doença Crônica Limite: Animals Idioma: En Revista: Am. j. trop. med. hyg Ano de publicação: 2006 Tipo de documento: Article