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Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum

Dussart, Phillipe; Labeau, Bhety; Louis, Philippe; Lagathu, Gisèle; Nunes, Marcio R. T.; Rodrigues, Suely G. Rodrigues; Storck-Herrman, Cécile; Cesaire, Raymond; Morvan, Jacques; Flamand, Marie; Baril, Laurence.
Clin. Vaccinne Immunol ; (Epub ahead of print): 1185-1189, 2006. tab, graf
Artigo em Inglês | BVS DIP, FIOCRUZ | ID: dip-2021
We evaluated a one-step sandwich-format microplate enzyme immunoassay for detecting dengue virus NS1 antigen in human serum, using Platelia(TM) Dengue NS1 Antigen (Ag) kits (Bio-Rad Laboratories, Marnes La Coquette, France). We collected 299 serum samples from patients with dengue disease and 50 serum samples from patients not infected with dengue virus. For the 239 serum samples from patients with acute infections testing positive by RT-PCR and/or virus isolation for one of the four dengue serotypes, the sensitivity of the Platelia(TM) Dengue NS1 Ag was 88.7% (95% confidence interval 84.0%-92.4%). None of the serum samples from patients not infected with dengue virus tested positive with the Platelia(TM) Dengue NS1 Ag kit. A diagnostic strategy combining Platelia(TM) Dengue NS1 Ag for acute-phase sera and MAC-ELISA for early convalescence-phase sera only increased sensitivity from 88.7% to 91.9%. Thus, NS1 antigen detection with the Platelia(TM) Dengue NS1 Ag kit could be used for first-line testing for acute dengue infection in clinical diagnostic laboratories...(AU)
Biblioteca responsável: BR275.1
Localização: BR275.1; PCIEC2006