Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil
Future microbiol
; 5(6): 971-980, 2010. mapas, tab, graf
Article
em En
| BVSDIP, FIOCRUZ
| ID: dip-3036
Biblioteca responsável:
BR275.1
Localização: BR275.1; PCIEC2010
ABSTRACT
Aim:
Our aim is to investigate if the clusters of postsurgical mycobacterialinfections, reported between 2004 and 2008 in seven geographically distantstates in Brazil, were caused by a single mycobacterial strain.MATERIALS &METHODS:
Available information from 929 surgical patients was obtained from local health authorities. A total of 152 isolates from surgical patients were identified by PCR restriction enzyme analysis of the hsp65 gene (PRA-hsp65) and sequencing of the rpoB gene. Isolates were typed by pulsed-field gel electrophoresis (PFGE) using two restriction enzymes, DraI and AseI. A total of 15 isolates not related to surgical cases were analyzed for comparison.RESULTS:
All isolates were identified as Mycobacterium abscessus ssp. massiliense. Isolates from surgical patients and one sputum isolate grouped in a single PFGE cluster, composed of two closely related patterns, with one band difference. A total of 14 other isolates unrelated to surgical cases showed distinctive PFGE patterns.CONCLUSION:
A particular strain of M. abscessus ssp. massiliense was associated with a prolonged epidemic of postsurgical infections in seven Brazilian states, suggesting that this strain may be distributed in Brazilian territory and better adapted to cause surgical-site infections.AU
Texto completo:
1
Base de dados:
BVSDIP
/
FIOCRUZ
Assunto principal:
Infecção Hospitalar
/
Mycobacterium
/
Infecções por Mycobacterium
Limite:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Child
/
Female
/
Humans
/
Male
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Future microbiol
Ano de publicação:
2010
Tipo de documento:
Article