Cellular and humoral immune responses against the Plasmodium vivax MSP-119 malaria vaccine candidate in individuals living in an endemic area in north-eastern Amazon region of Brazil
The prevalence of activated CD4+ was greater than CD8+ T cells, in both ex-vivo and in 96 h culture in presence of PvMSP-119 and PSS1 antigen. A low proliferative response against PvMSP-119 and PSS1 crude antigen after 96 h culture was observed. High plasmatic levels of IFN-? and IL-10 as well as lower TNF levels were also detected in malariapatients. However, in the 96 h supernatant culture, the dynamics of cytokine responses differed from those depicted on plasma assays; in presence of PvMSP-119 stimulus, higher levels of TNF were noted in supernatant 96 h culture of malariapatientscells while low levels of IFN-? and IL-10 were verified. High frequency of malariapatients presenting antibodies against PvMSP-119 was evidenced, regardless class or IgG subclass. PvMSP-119-induced antibodies were predominantly on non-cytophilic subclasses.
Conclusions:
The results presented here shows that PvMSP-119 was able to induce a... (AU)