Chromosome 17 abnormalities and mutation of the TP53 gene: correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
Genet. mol. biol
; 28(1): 40-43, Jan.-Mar. 2005. ilus, tab
Article
em En
| LILACS
| ID: lil-399613
Biblioteca responsável:
BR26.1
RESUMO
Alterations involving the short arm of chromosome 17 (17p) during the progression of chronic myeloid leukemia (CML) have been described. This chromosomal region contains the tumor suppressor gene TP53 that may be an important factor in the evolution of this disease. In this study, we used flow cytometry and western blotting to assess p53 protein expression and single stranded conformational polymorphism to examine TP53 gene alterations in three patients with CML who showed alterations in 17p. Only the case with del(17)(p11) had p53 expression positive by flow cytometry and an abnormal migration pattern by SSCP analysis. The importance of the correlation between the results obtained with these techniques, as well as the clinical course of the patients, are discussed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Leucemia Mielogênica Crônica BCR-ABL Positiva
/
Aberrações Cromossômicas
Tipo de estudo:
Etiology_studies
Limite:
Humans
Idioma:
En
Revista:
Genet. mol. biol
Assunto da revista:
GENETICA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Brasil