Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis
J. venom. anim. toxins incl. trop. dis
; 2004/02/2014.
Article
em En
| LILACS
| ID: lil-724683
Biblioteca responsável:
BR68.1
ABSTRACT
Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect the sensitivity of PCR assays, including DNA yield and purity, an optimal extraction method is pivotal. Losses of a parasites DNA during extraction may significantly impair its detection by PCR and lead to false-negative results. This study proposes a triplex PCR assay targeting the parasites DNA, an external control (pUC18) and an internal control (G3PD) for accurate diagnosis of leishmaniasis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Leishmaniose
/
Reação em Cadeia da Polimerase
/
Diagnóstico
/
Reações Falso-Negativas
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J. venom. anim. toxins incl. trop. dis
Assunto da revista:
TOXICOLOGIA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Brasil