Differential expression of stem cell-like proteins in normal, hyperplastic and dysplastic oral epithelium
J. appl. oral sci
; 23(1): 79-86, Jan-Feb/2015. tab, graf
Article
em En
| LILACS, BBO
| ID: lil-741593
Biblioteca responsável:
BR1.1
ABSTRACT
Objective The identification of stem cells (SC) remains challenging. In the human oral mucosal epithelium, these cells are believed to be in the basal layer (stem cell niche), but their exact location is unclear. The aim of this study was to examine the dysplastic oral epithelium for these SC-like proteins in order to assess their diagnostic value as biomarkers complementing the histological grading of dysplasia. Material and Methods Thirty oral epithelial dysplasia (OED), 25 oral lichen planus (OLP), 10 oral hyperkeratosis and 5 normal oral epithelium (OE) were immunohistochemically examined for four SC markers [integrin β1, neuron-glial-2 (NG2), notch 1 (N1) and keratin 15 (K15)]. Results Three of four SC markers were heterogeneously detected in all samples. K15 overexpression in the lower two-thirds of severe OED suggests an expanded SC niche. Integrin β1 distribution pattern was not measurably different between OEDs and control. NG2 was almost negative to absent in all samples examined. N1 expression was weak and highly variable in normal and dysplastic epithelium, making it an unreliable epithelial stem cell marker. Conclusions Present findings suggest that these markers were unable to identify individual epithelial stem cells. Instead, subpopulations of cells, most probably stem cells and transit amplifying cells with stem cell-like properties were identified in the dysplastic oral epithelium. The characteristic expressions of K15 might be of diagnostic value for oral dysplasia and should be investigated further. .
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
BBO
/
LILACS
Assunto principal:
Células-Tronco
/
Proteínas
/
Células Epiteliais
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
J. appl. oral sci
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Malásia