Syndecan-2 induces filopodia by active cdc42Hs.
Exp Cell Res
; 248(2): 439-56, 1999 May 01.
Article
em En
| MEDLINE
| ID: mdl-10222136
ABSTRACT
The syndecans, a family of transmembrane heparan sulfate proteoglycans, are ubiquitous molecules whose intracellular function is still unknown. To examine the function of syndecan-2, one of the most abundant heparan sulfate proteoglycan in fibroblasts, we performed transfection studies in COS-1 and Swiss 3T3 cells. Endogenous syndecan-2 colocalized with F-actin in cortical structures. Overexpression of full-length syndecan-2 induced the formation of long filopodia-like structures. These changes correlated with a rearrangement of the actin cytoskeleton, which strongly colocalized with syndecan-2. Overexpression of syndecan-2 lacking the extracellular domain increased the number of microspikes on the cell surface but failed to induce filopodia. Addition of heparin blocked the effect of full-length syndecan-2, suggesting that heparan sulfate chains in the extracellular domain are necessary to induce filopodia. Coexpression of cdc42Hs negative-dominant N17 blocked syndecan-2-induced filopodia and cdc42Hs positive-dominant V12 had a synergic effect. This indicates that active cdc42Hs is necessary for syndecan-2 induction of filopodia. These results provide a link between syndecan-2, actin cytoskeleton, and cdc42Hs.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteoglicanas
/
Pseudópodes
/
Citoesqueleto
/
Glicoproteínas de Membrana
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Proteínas de Ciclo Celular
/
Proteínas de Ligação ao GTP
Limite:
Animals
Idioma:
En
Revista:
Exp Cell Res
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Espanha