Comparison of four derivatizing reagents for 6-acetylmorphine GC-MS analysis.
J Anal Toxicol
; 23(4): 262-9, 1999.
Article
em En
| MEDLINE
| ID: mdl-10445489
ABSTRACT
The propionyl, trimethylsilyl, trifluroacetyl, and heptafluoroacyl derivatives of 6-acetylmorphine (6-AM) were evaluated with respect to optimal method performance, derivative stability, and methods characterization for use in gas chromatographic-mass spectrometric (GC-MS) analysis with electron ionization mode and selected ion monitoring. The most common potential interferences and compatibility with other derivatives when used on the same GC-MS were determined for the derivatizing reagents. The propionyl, trimethylsilyl, and trifluroacetyl derivatives produced adequate stability, accuracy, and precision for the method. The 6-AM derivatization with commercially available propionic anhydride generated a relatively small amount of 6-AM-propionyl derivative from the free morphine present in a specimen. The trimethylsilyl derivative obtained by the reaction with MSTFA did not require incubation, was the easiest to prepare, and had the highest potential for use on an automated sample-preparation device. An important advantage of derivatization with MSTFA is elimination of the possibility of heroin decomposition to 6-AM that is due to incubation at elevated temperature.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Detecção do Abuso de Substâncias
/
Derivados da Morfina
Limite:
Humans
Idioma:
En
Revista:
J Anal Toxicol
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Estados Unidos