Requirement of the inositol trisphosphate receptor for activation of store-operated Ca2+ channels.
Science
; 287(5458): 1647-51, 2000 Mar 03.
Article
em En
| MEDLINE
| ID: mdl-10698739
ABSTRACT
The coupling mechanism between endoplasmic reticulum (ER) calcium ion (Ca2+) stores and plasma membrane (PM) store-operated channels (SOCs) is crucial to Ca2+ signaling but has eluded detection. SOCs may be functionally related to the TRP family of receptor-operated channels. Direct comparison of endogenous SOCs with stably expressed TRP3 channels in human embryonic kidney (HEK293) cells revealed that TRP3 channels differ in being store independent. However, condensed cortical F-actin prevented activation of both SOC and TRP3 channels, which suggests that ER-PM interactions underlie coupling of both channels. A cell-permeant inhibitor of inositol trisphosphate receptor (InsP3R) function, 2-aminoethoxydiphenyl borate, prevented both receptor-induced TRP3 activation and store-induced SOC activation. It is concluded that InsP3Rs mediate both SOC and TRP channel opening and that the InsP3R is essential for maintaining coupling between store emptying and physiological activation of SOCs.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Canais de Cálcio
/
Cálcio
/
Receptores Citoplasmáticos e Nucleares
/
Sinalização do Cálcio
/
Retículo Endoplasmático
Limite:
Humans
Idioma:
En
Revista:
Science
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Estados Unidos