Antifluorescein affinity columns. Isolation and immunocompetence of lymphocytes that bind fluoresceinated antigens in vivo or in vitro.
J Exp Med
; 144(1): 69-78, 1976 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-1084410
ABSTRACT
A new method for the isolation of specific immunocompetent lymphocytes has been described in which lymphocyte populations are exposed to fluoresceinated antigens (FLAGs) in vivo or in vitro, and the FLAG-binding cells retained on antifluorescein affinity columns. Specific cells are then eluted with an unrelated FL-labeled protein and shown to be fully immunocompetent. This methodology has been applied successfully in diverse antigenic systems including polymerized flagellin and TNP-specific B cells and alloantigen-reactive cytotoxic T lymphocytes. The method is rapid, inexpensive (requiring only antifluorescein beads), and can be applied to any antigens (or antibodies) in which a fluorescein group can be introduced.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos
Limite:
Animals
Idioma:
En
Revista:
J Exp Med
Ano de publicação:
1976
Tipo de documento:
Article