Rapid differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi sensu stricto by LightCycler fluorescence melting curve analysis of a PCR product of the recA gene.
J Clin Microbiol
; 38(7): 2756-9, 2000 Jul.
Article
em En
| MEDLINE
| ID: mdl-10878080
ABSTRACT
To differentiate the Borrelia burgdorferi sensu lato genospecies, LightCycler real-time PCR was used for the fluorescence (SYBR Green I) melting curve analysis of borrelial recA gene PCR products. The specific melting temperature analyzed is a function of the GC/AT ratio, length, and nucleotide sequence of the amplified product. A total of 32 DNA samples were tested. Of them three were isolated from B. burgdorferi reference strains and 16 were isolated from B. burgdorferi strains cultured from Ixodes ricinus ticks; 13 were directly isolated from nine human biopsy specimens and four I. ricinus tick midguts. The melting temperature of B. garinii was 2 degrees C lower than that of B. burgdorferi sensu stricto and B. afzelii. Melting curve analysis offers a rapid alternative for identification and detection of B. burgdorferi sensu lato genospecies.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Recombinases Rec A
/
Borrelia
/
Doença de Lyme
/
Grupo Borrelia Burgdorferi
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Reação em Cadeia da Polimerase
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Borrelia burgdorferi
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Finlândia