Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments.
Proc Natl Acad Sci U S A
; 72(4): 1344-8, 1975 Apr.
Article
em En
| MEDLINE
| ID: mdl-1093178
ABSTRACT
Cytoplasmic RNA, isolated from cells late after infection by adenovirus type 2 and fractionated by hybridization to specific fragments of adenovirus DNA produced by cleavage with the endonuclease R-EcoRI, was used as template for protein synthesis in cell-free mammalian extracts. Each of the R-EcoRI fragments of DNA selects RNA that encodes specific subsets of the viral polypeptides. From the known order of the R-EcoRI fragments, the following partial map is deduced (III, IIIa, IVa2, V, P-VII, IX), (II, P-VI), 100K, IV-where the relative order of the components enclosed in parentheses has not yet been determined.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biossíntese de Proteínas
/
DNA Viral
/
Adenoviridae
/
Genes
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
1975
Tipo de documento:
Article