PCR-based subtractive hybridization identifies repressed genes in growth-arrested human dermal fibroblasts following combined treatment with 8-methoxypsoralen and UVA irradiation (PUVA).
Exp Gerontol
; 35(5): 573-81, 2000 Aug.
Article
em En
| MEDLINE
| ID: mdl-10978679
ABSTRACT
To identify genes which are repressed in growth-arrested human dermal fibroblasts upon a single treatment with 8-methoxypsoralen and UVA irradiation (PUVA) we have used a PCR-based subtractive hybridization protocol resulting in cloning of four PUVA-repressed genes. Sequence analysis and homology searches identified three known genes related to growth control, lipid and connective tissue metabolism. One cDNA clone represented a novel gene. Northern blot analyses confirmed a PUVA-dependent reduction in mRNA expression in fibroblasts in vitro. The identification of growth arrest related repressed genes in PUVA-treated fibroblasts may stimulate further research addressing the causal role of these genes in the control and regulation of the postmitotic phenotype of fibroblasts on a molecular and cellular level.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Terapia PUVA
/
Pele
/
Senescência Celular
Tipo de estudo:
Guideline
Limite:
Child
/
Child, preschool
/
Humans
/
Male
Idioma:
En
Revista:
Exp Gerontol
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Alemanha