The Chemical Shift Index method applied to resin-bound peptides.
J Pept Res
; 56(6): 346-51, 2000 Dec.
Article
em En
| MEDLINE
| ID: mdl-11152293
ABSTRACT
The Chemical Shift Index (CSI) method proposed by Wishart et al. [Biochemistry (1992) 31, 1647-1651] to evaluate the secondary structure of peptides in aqueous solution uses as its reference the chemical shift values of each of the 20 natural amino acids (X) in a typical nonstructured sequence GGXAGG (17-20). In order to apply the CSI method to protected resin-bound peptides, we established a new database of chemical shift values for the same GGXAGG sequences in their protected form and anchored to a polystyrene resin swollen in DMF-d7. The predictive value of this new reference set in the CSI protocol was tested on different resin-bound peptides that were previously characterized by a full NOE analysis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Resinas Vegetais
/
Espectroscopia de Ressonância Magnética
/
Técnicas de Química Analítica
/
Modelos Químicos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Pept Res
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
França