Stabilization of Penicillium occitanis cellulases by spray drying in presence of Maltodextrin.
Enzyme Microb Technol
; 28(2-3): 253-258, 2001 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-11166820
ABSTRACT
The stabilization of fungal cellulases by spray drying, the thermal stability of Penicillium occitanis cellulases and the effect of some additives were studied. We observed that CMCase activity presents a good stability at 50 degrees C, even after 60 h of incubation. On the other hand, beta-glucosidase activity was more sensitive (loss of 50%) and reacts on total cellulases activities (Filter paper activities). The addition of hydrophilic agents such as ethylene glycol, polyethylene glycol (PEG6000) enhanced enzyme activity. The effect of PEG and Maltodextrin, another water activity decreasing agent, were then tested during the spray drying of Pol6 cellulases. The presence of 1% PEG allowed the best recovery but had a negative effect on enzyme stability while 1% Maltodextrin showed a negative effect on enzyme recovery but a very positive effect on enzyme stabilization.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Enzyme Microb Technol
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Tunísia