LC method for the analysis of acetylsalicylic acid, caffeine and codeine phosphate in pharmaceutical preparations.
J Pharm Biomed Anal
; 25(1): 93-101, 2001 Apr.
Article
em En
| MEDLINE
| ID: mdl-11274862
ABSTRACT
An accurate, simple, reproducible and sensitive method for the determination of acetylsalicylic acid, caffeine and codeine phosphate has been developed and validated. Acetylsalicylic acid, caffeine and codeine phosphate were separated using a microBondapack C(8) column by isocratic elution with flow rate 1.0 ml/min. The mobile phase composition was 125/125/250/0.5 (v/v) isopropyl alcohol, acetonitrile, water and o-phosphoric acid. The samples were detected at 215 nm using photo-diode array detector. The linear range of detection for acetylsalicylic acid, caffeine and codeine phosphate were between 0.40 and 1000, 0.25 and 250, and 0.48 and 96 microg/ml, respectively. The linearity, range, selectivity, system performance parameters, precision, accuracy, and ruggedness for acetylsalicylic acid, caffeine and codeine phosphate were also shown to have acceptable values.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cafeína
/
Aspirina
/
Cromatografia Líquida de Alta Pressão
/
Codeína
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Turquia