Distribution and viral load of type specific HPVs in different cervical lesions as detected by PCR-ELISA.
J Clin Pathol
; 54(5): 377-80, 2001 May.
Article
em En
| MEDLINE
| ID: mdl-11328837
ABSTRACT
AIMS:
To investigate the distribution and viral load of the most prevalent high risk human papillomavirus (HPV) types 16, 18, 31, 33, and 45 and low risk HPV types 6 and 11 in a variety of cervical lesions.METHODS:
One hundred and seventy six cytological specimens from women with different cervical lesions were investigated. For an accurate standardisation of the sample, cervical cells were counted and a volume of the cell suspension processed by polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA). Semiquantitative determinations were achieved in relation to an external reference titration curve.RESULTS:
HPV DNA was detected in 60.2% of the samples. HPV-16 was the prevalent genotype (57.6%), followed by HPV-33, HPV-31, HPV-6, HPV-18, and HPV-45. HPV-11 was not detected. HPV-16 showed a pronounced increase in prevalence with the evolution of cervical disease. Semiquantitative evaluation of the results showed that only HPV-16 DNA could reach very high values (> 1000 genome copies/cell) and a very high HPV-16 load correlated with the severity of cervical disease.CONCLUSIONS:
Only HPV-16 load appears to be associated with the severity of cervical disease.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Papillomaviridae
/
Displasia do Colo do Útero
/
Neoplasias do Colo do Útero
/
Carga Viral
Tipo de estudo:
Risk_factors_studies
Limite:
Adult
/
Aged
/
Female
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Humans
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Middle aged
Idioma:
En
Revista:
J Clin Pathol
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Itália