Snf1--a histone kinase that works in concert with the histone acetyltransferase Gcn5 to regulate transcription.
Science
; 293(5532): 1142-6, 2001 Aug 10.
Article
em En
| MEDLINE
| ID: mdl-11498592
ABSTRACT
Modification of histones is an important element in the regulation of gene expression. Previous work suggested a link between acetylation and phosphorylation, but questioned its mechanistic basis. We have purified a histone H3 serine-10 kinase complex from Saccharomyces cerevisiae and have identified its catalytic subunit as Snf1. The Snf1/AMPK family of kinases function in conserved signal transduction pathways. Our results show that Snf1 and the acetyltransferase Gcn5 function in an obligate sequence to enhance INO1 transcription by modifying histone H3 serine-10 and lysine-14. Thus, phosphorylation and acetylation are targeted to the same histone by promoter-specific regulation by a kinase/acetyltransferase pair, supporting models of gene regulation wherein transcription is controlled by coordinated patterns of histone modification.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Quinases
/
Saccharomyces cerevisiae
/
Proteínas Fúngicas
/
Histonas
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Regulação Fúngica da Expressão Gênica
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Ativação Transcricional
/
Proteínas Serina-Treonina Quinases
/
Proteínas de Saccharomyces cerevisiae
/
Proteínas de Ligação a DNA
Idioma:
En
Revista:
Science
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Estados Unidos