Production of beta-defensin-2 by human colonic epithelial cells induced by Salmonella enteritidis flagella filament structural protein.
FEBS Lett
; 508(3): 484-8, 2001 Nov 23.
Article
em En
| MEDLINE
| ID: mdl-11728477
ABSTRACT
We recently showed that FliC of Salmonella enteritidis increased human beta-defensin-2 (hBD-2) expression, and now describe the signaling responsible pathway. FliC increased the intracellular Ca(2+) concentration ([Ca(2+)](in)) in Caco-2 cells. The [Ca(2+)](in) increase induced by FliC was prevented by U73122 and heparin, but not by chelating extracellular Ca(2+) or pertussis toxin. The FliC-induced increase in hBD-2 promoter activity via nuclear factor kappaB (NF-kappaB) was also inhibited by chelation of intracellular Ca(2+) or by U73122. We conclude that FliC increased [Ca(2+)](in) via inositol 1,4,5-trisphosphate, which was followed by up-regulating hBD-2 mRNA expression via an NF-kappaB-dependent pathway.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Salmonella enteritidis
/
Beta-Defensinas
/
Flagelina
/
Mucosa Intestinal
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Japão