The GM130 and GRASP65 Golgi proteins cycle through and define a subdomain of the intermediate compartment.
Nat Cell Biol
; 3(12): 1101-13, 2001 Dec.
Article
em En
| MEDLINE
| ID: mdl-11781572
ABSTRACT
Integrating the pleomorphic membranes of the intermediate compartment (IC) into the array of Golgi cisternae is a crucial step in membrane transport, but it is poorly understood. To gain insight into this step, we investigated the dynamics by which cis-Golgi matrix proteins such as GM130 and GRASP65 associate with, and incorporate, incoming IC elements. We found that GM130 and GRASP65 cycle via membranous tubules between the Golgi complex and a constellation of mobile structures that we call late IC stations. These stations are intermediate between the IC and the cis-Golgi in terms of composition, and they receive cargo from earlier IC elements and deliver it to the Golgi complex. Late IC elements are transient in nature and sensitive to fixatives; they are seen in only a fraction of fixed cells, whereas they are always visible in living cells. Finally, late IC stations undergo homotypic fusion and establish tubular connections between themselves and the Golgi. Overall, these features indicate that late IC stations mediate the transition between IC elements and the cis-Golgi face.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glicoproteínas de Membrana
/
Compartimento Celular
/
Complexo de Golgi
/
Proteínas de Membrana
Limite:
Animals
Idioma:
En
Revista:
Nat Cell Biol
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Itália