Increased expression of endothelin-converting enzyme-1 in failing human myocardium.
Clin Sci (Lond)
; 103 Suppl 48: 237S-240S, 2002 Aug.
Article
em En
| MEDLINE
| ID: mdl-12193094
ABSTRACT
Endothelin-1 (ET-1) is considered to be involved in the development and progression of heart failure. Therefore, we analysed the expression of endothelin-converting enzyme-1 (ECE-1), endothelin receptors A (ET(A)) and B (ET(B)) mRNAs by standard-calibrated, competitive reverse transcriptase-PCR using an internal-deleted in vitro-transcribed cRNA standard. ET-1 peptide levels were measured using isoform-specific rabbit antibodies against synthetic ET-1. mRNA and protein expression was determined in the right atrial myocardium of New York Heart Association class I patients and class IV patients undergoing aorto-coronary bypass surgery. ECE-1 mRNA was upregulated in failing atrial myocardium. Furthermore, ET-1 peptide levels were increased in failing atrial myocardium. Atrial ET(A) mRNA expression was not changed, while ET(B) mRNA was downregulated in the failing atrial myocardium. Our results support an upregulation of ET-1 synthesis by induction of ECE-1 in failing atrial myocardium. Pharmacological inhibition of augmented ECE-1 expression might provide a new therapeutic perspective in the treatment of heart failure.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Ácido Aspártico Endopeptidases
/
Insuficiência Cardíaca
/
Miocárdio
Limite:
Humans
Idioma:
En
Revista:
Clin Sci (Lond)
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Alemanha