[Separation and determination of 1,5-benzothiazepines-alpha-chloro-beta-lactam with thin-layer chromatography].

ABSTRACT

A method for separation and determination of 1,5-benzothiazepines-alpha-chloro-beta-lactam in reaction mixture by thin-layer chromatography has been established. The operating conditions were lambda 1 = 270 nm, lambda 2 = 310 nm, beam size 1.2 mm x 1.2 mm, SX = 3. The calibration curve was linear in the range of 0.285-5.7 micrograms (r = 0.9952, n = 8) and the detectable limit was 0.114 microgram. The coefficient of variation of this method was less than 5% and the average recovery for beta-lactam was 96.75%. The method is simple and rapid.