Proteolytic activity of a yeast cell wall lytic Arthrobacter species.
Lett Appl Microbiol
; 36(4): 227-9, 2003.
Article
em En
| MEDLINE
| ID: mdl-12641716
ABSTRACT
AIMS:
To investigate properties of the proteolytic activity of a yeast cell wall lytic soil bacterium identified as an Arthrobacter species. METHODS ANDRESULTS:
The organism was grown at pH 7.5 and 30 degrees C in shake flasks on media with different complex subtrates. Highest proteolytic activity assayed with azocaseine was detected in media with wheat gluten. In addition, l-leucine, l-alanine exopeptidase activity and esterase activity were found. The proteolytic activity showed stability up to pH 12, with a maximum at pH 11. The temperature optimum was at 55 degrees C, but there was a loss in enzyme activity of 50% within 2 h. The proteolytic activity was inhibited by 3,4-dichloroisocumarin, whereas there was little or no effect with EDTA, pepstatin A or E64.CONCLUSIONS:
The proteolytic activity is highly alkaline stable. The formation of the enzyme can be induced by media with high protein content.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arthrobacter
Idioma:
En
Revista:
Lett Appl Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Áustria