Dynamic regulation of expression of colony-stimulating factor 1 in the reproductive tract of cattle during the estrous cycle and in pregnancy.
Biol Reprod
; 69(2): 518-28, 2003 Aug.
Article
em En
| MEDLINE
| ID: mdl-12672668
ABSTRACT
Colony-stimulating factor 1 (CSF-1) is a hematopoetic cytokine that also plays an important role in placental physiology. We report here the molecular cloning of two alternative splice variants of the bovine gene coding for a putative secreted and a membrane-bound form of the cytokine and the dynamic regulation of expression in the reproductive tract of cattle during the estrous cycle and pregnancy. Bovine CSF-1 was expressed mainly as the 3- and 4-kilobase (kb) transcripts, but 1.4- and 0.8-kb mRNAs were also detected in Day 50-70 pregnant uterine tissue. During the estrous cycle, both the 4- and 3-kb mRNAs were present, but the 3-kb putative membrane-bound form was more abundant than the 4-kb secreted form during diestrus. This pattern of expression was reversed in pregnancy, so that the exponential increase in CSF-1 expression seen during pregnancy was due predominantly to increased abundance of the 4-kb transcript. The change in the 4-kb3-kb ratio was detected between Day 14 and Day 17, approximately the time of maternal recognition of pregnancy. Thus, CSF-1 was identified as one gene whose expression in the uterus might be altered early in response to the presence of the conceptus. CSF-1 was also expressed in the extraembryonic membranes of the conceptus and in the trophoblastic cells of the fetal cotyledons after the formation of the placentomes. The high level of CSF-1 expression during bovine pregnancy in uteroplacental tissues is consistent with its proposed role in placental physiology.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Prenhez
/
Fator Estimulador de Colônias de Macrófagos
/
Regulação da Expressão Gênica no Desenvolvimento
/
Ciclo Estral
/
Genitália Feminina
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Biol Reprod
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Nova Zelândia