Evidence of aromatase localization in cytoplasmic droplet of human immature ejaculated spermatozoa.
Folia Histochem Cytobiol
; 41(1): 23-7, 2003.
Article
em En
| MEDLINE
| ID: mdl-12705475
ABSTRACT
Lytochrome P450 aromatase is a microsomal enzyme catalyzing the conversion of androgens to estrogens. P450arom expression has been demonstrated in testicular and epididymal sperm cells of several species but very limited data have been reported about maturating human germ cells. In this study, human spermatozoa with cytoplasmic droplet anomaly have been utilized to investigate aromatase immunolocalization in the immature germ cells of human ejaculate. Immunodetection has utilized a polyclonal antiserum as primary antibody, a biotinylated IgG as secondary antibody and then the avidin-biotin-peroxidase complex amplification followed by the diaminobenzidine staining. A strong immunoreaction was observed in the cytoplasmic droplets retained around the midpiece of immature spermatozoa and also in the descending droplets of late maturing sperm, while the other cellular components were unstained. Therefore, this investigation has demonstrated, for the first time, aromatase immunolocalization in residual cytoplasm of human ejaculated sperm, suggesting cytoplasmic droplets as possible estrogen biosynthesis sites during human sperm differentiation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espermatozoides
/
Aromatase
/
Citoplasma
Limite:
Humans
/
Male
Idioma:
En
Revista:
Folia Histochem Cytobiol
Assunto da revista:
HISTOCITOQUIMICA
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Itália