[Inhibitory effect of artemin on endotoxin-induced nitric oxide synthesis].
Zhongguo Zhong Yao Za Zhi
; 26(11): 770-3, 2001 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-12776351
ABSTRACT
OBJECTIVE:
To investigate the effect of Artemin on LPS-induced nitric oxide synthesis in macrophages.METHOD:
1. Nitrite oxide (NO) production of RAW 264.7 cells was induced by LPS or LPS in combination with interferon-gamma (IFN gamma) in the presence or absence of Artemin. The amount of NO in the supernatant of RAW 264.7 cells was detected with Griess reagent. 2. Balb/c mice were injected with Artemin (i.m.) 50 mg.kg-1.d-1 x 3 d, and intraperitoneal macrophages were collected to detect the LPS-induced NO production.RESULT:
LPS 1.0, 0.2 microgram.ml-1 or IFN-gamma 100u + LPS 1.0, 0.2, 0.04 microgram.ml-1 could induced a large amount of NO synthesis of RAW 264.7 cells. Artemin showed a significant inhibitory effect on LPS or IFN-gamma + LPS-induced NO production in a dose dependent manner. After treatment with Artemin, the response of Balb/c mice to LPS was reduced, which was showed by a decrease in NO production of intraperitoneal macrophages induced by LPS.CONCLUSION:
Artemin could reduce LPS-induced production of inflammatory factors resulting in the inhibition of inflammatory effects.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Macrófagos Peritoneais
/
Proteínas do Tecido Nervoso
/
Óxido Nítrico
Limite:
Animals
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Assunto da revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
China