Binding specificities of the mismatch binding protein, MutS, to oligonucleotides containing modified bases.
Nucleic Acids Res Suppl
; (1): 221-2, 2001.
Article
em En
| MEDLINE
| ID: mdl-12836344
ABSTRACT
We have studied the effects of DNA mismatch repair on mutagenesis induced by nucleoside analogs. Among them, the mutagenic action of 3,4-dihydro-6H,8H-pyrimido[4,5-c][1,2]oxazin-7-one 2'-deoxyriboside (dP) showed high susceptibility to the mismatch repair system, while mutagenesis by N4-aminocytidine and N4-hydroxycytidine was only weakly affected. 2-Aminopurine mutagenesis showed intermediate susceptibility. MutS protein specifically bound to an oligonucleotide duplex containing a dP-dG pair, while the dP-dA pair was bound only weakly. The binding to the dP-dG pair was as strong as binding to a dA-dC mismatch. These specific binding properties can explain the effective avoidance of dP-induced mutagenesis by the mismatch repair system. We have also studied the effects of the repair system on mutagenesis induced by methylating and ethylating agents.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Proteínas de Bactérias
/
Adenosina Trifosfatases
/
Citidina
/
Proteínas de Escherichia coli
/
Proteínas de Ligação a DNA
/
Ácidos Nucleicos Heteroduplexes
Idioma:
En
Revista:
Nucleic Acids Res Suppl
Assunto da revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Ano de publicação:
2001
Tipo de documento:
Article