Protein-DNA interaction mapping using genomic tiling path microarrays in Drosophila.
Proc Natl Acad Sci U S A
; 100(16): 9428-33, 2003 Aug 05.
Article
em En
| MEDLINE
| ID: mdl-12876199
ABSTRACT
We demonstrate the use of a chromosomal walk (or "tiling path") printed as DNA microarrays for mapping protein-DNA interactions across large regions of contiguous genomic DNA in Drosophila melanogaster. Microarrays were constructed with genomic DNA fragments 430-920 bp in length, covering 2.9 million base pairs of the Adh-cactus region of chromosome 2 and 85,000 base pairs of the 82F region of chromosome 3. We performed DNA localization mapping for the heterochromatin protein HP1 and for the sequence-specific GAGA transcription factor, producing a comprehensive, high-resolution map of in vivo protein-DNA interactions throughout these regions of the Drosophila genome.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Proteínas de Ligação a DNA
/
Drosophila
Limite:
Animals
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Estados Unidos