Endogenous Mtv-8 or a closely linked sequence stimulates rearrangement of the downstream V kappa 9 gene.
J Immunol
; 149(4): 1242-51, 1992 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-1323611
ABSTRACT
Mtv-8 is an endogenous retrovirus located 4.6 kb upstream of a V kappa region gene (called V kappa 9M) within the kappa-Ig locus. The proximity of these two genes resulted in several effects. Using a newly developed RNase protection assay for measuring transcription from a single endogenous provirus, we found that Mtv-8 transcription could be detected after juxtaposition of the kappa-enhancers to the normally silent provirus. Reciprocally, using the polymerase chain reaction we observed that the frequency of V kappa 9M rearrangement was 5- to 10-fold higher in spleens from Mtv-8-positive mice (BALB/c, C58.C, A/J, and B6) compared to spleens from mice that lacked the Mtv-8 provirus (C58, C.C58, NZB, and PERA/Ei). Molecular cloning and sequencing of the V kappa 9M gene from C.C58 mice (containing the kappa-locus from C58 mice on a BALB/c background) indicated that at least some Mtv-8-negative strains have a functional V kappa 9M gene. Together these data suggest that Mtv-8 or a closely linked gene enhances V kappa 9M rearrangement. Since Mtv-8 also reportedly produces a superantigen, it appears that Mtv-8 may influence both the T cell and B cell repertoires.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Viral
/
Região Variável de Imunoglobulina
/
Genes de Imunoglobulinas
/
Linfócitos B
/
Rearranjo Gênico de Cadeia Leve de Linfócito B
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Cadeias kappa de Imunoglobulina
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Vírus da Leucemia Murina
Limite:
Animals
Idioma:
En
Revista:
J Immunol
Ano de publicação:
1992
Tipo de documento:
Article