Nucleotide sequence of the rhaR-sodA interval specifying rhaT in Escherichia coli.
J Gen Microbiol
; 138(6): 1109-16, 1992 Jun.
Article
em En
| MEDLINE
| ID: mdl-1339463
ABSTRACT
The precise location of the rhaT gene, encoding rhamnose permease, has been established between sodA and rhaC at 3605-3607 kb of Kohara's physical map, which corresponds to 88.4 min on the Escherichia coli chromosomal map. The dependence of the activity of the rhaT product on the function of rhaC, the rhamnose operon regulatory gene, was established by measuring rhamnose transport in wild-type and rhaC-deficient strains. The sequence of the sodA-rhaC interval displayed a single ORF corresponding to rhaT, which is transcribed counterclockwise on the E. coli chromosome. The ORF was shown to be preceded by a ribosome binding consensus sequence and a catabolite repression protein consensus sequence. The derived amino acid sequence displayed very low homology with any other permease and was clearly dissimilar to the homologous group formed by the xylose, arabinose, galactose and several glucose transporters. Analysis of the rhaT primary sequence identified potential membrane-spanning regions, possibly defining a protein structure model different from the one corresponding to the above-mentioned homologous group.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
/
Ramnose
/
Proteínas de Escherichia coli
/
Simportadores
/
Escherichia coli
Idioma:
En
Revista:
J Gen Microbiol
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Espanha