Electrotransformation of intact cells of Brevibacterium flavum MJ-233.
J Ind Microbiol
; 5(2-3): 159-65, 1990.
Article
em En
| MEDLINE
| ID: mdl-1366679
ABSTRACT
Electroporation allowed transformation of intact cells of Brevibacterium flavum MJ-233. The two plasmids used for electroporation were pCRY2 (6.3 kilobase) and pCRY3 (8.2 kilobases). Both plasmids contain the chloramphenicol-resistance gene and the autonomous replication origin in B. flavum MJ-233. The efficiency of electrotransformation was optimal with cells harvested at the middle log phase of growth, and was improved by the addition of 1.0 U/ml of penicillin G to the culture medium. The optimum yield of transformants per micrograms DNA was 5 x 10(4) when the cell suspension was pulsed at a cell density of 1 x 10(10)/ml and at a DNA amount of 1.0 micrograms.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Transformação Bacteriana
/
Brevibacterium
/
DNA Bacteriano
Idioma:
En
Revista:
J Ind Microbiol
Assunto da revista:
BIOTECNOLOGIA
/
MICROBIOLOGIA
Ano de publicação:
1990
Tipo de documento:
Article
País de afiliação:
Japão