A novel, quantitative bioassay for type I interferon using a recombinant indicator cell line.
Biotechnology (N Y)
; 8(12): 1263-7, 1990 Dec.
Article
em En
| MEDLINE
| ID: mdl-1366960
ABSTRACT
We describe a specific and quantitative novel assay for biologically active human type I interferon (IFN), the MxR assay. It is based on a Vero cell line containing multiple copies of a hybrid gene consisting of the murine Mx promoter, which is responsive to type I IFN, linked to the human growth hormone (hGH) transcription unit. Exposure of this cell line to IFN-alpha or -beta for 12-48 hours results in the production of hGH that is measured by a commercially available radio-immune assay. The response to IFN-alpha is dose-dependent between 3 and 1000 units/ml. There is no response to TNF, IL-1 and a number of other cytokines and growth factors, and only a negligible response to IFN-gamma.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bioensaio
/
Interferon Tipo I
/
Proteínas de Ligação ao GTP
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biotechnology (N Y)
Ano de publicação:
1990
Tipo de documento:
Article
País de afiliação:
Suíça