Induction of broadly cross-reactive cytotoxic T cells recognizing an HIV-1 envelope determinant.
Science
; 255(5042): 333-6, 1992 Jan 17.
Article
em En
| MEDLINE
| ID: mdl-1372448
ABSTRACT
An immunodominant determinant for cytotoxic T lymphocytes (CTLs) exists in the hypervariable portion of human immunodeficiency virus-1 (HIV-1) gp160. Three mouse CTL lines (specific for isolates MN, RF, and IIIB) were examined for recognition of homologous determinants from distinct isolates. Only MN-elicited CTLs showed extensive interisolate cross-reactivity. Residue 325 played a critical role in specificity, with MN-elicited CTLs responding to peptides with an aromatic or cyclic residue and IIIB-induced cells recognizing peptides with an aliphatic residue at this position. CTL populations with broad specificities were generated by restimulation of IIIB-gp160 primed cells with MN-type peptides that have an aliphatic substitution at 325. This represents an approach to synthetic vaccines that can generate broadly cross-reactive CTLs capable of effector function against a wide range of HIV isolates.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Precursores de Proteínas
/
Linfócitos T Citotóxicos
/
Produtos do Gene env
/
HIV-1
Limite:
Animals
Idioma:
En
Revista:
Science
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Japão