Low response of BALB/c macrophages to priming and activating signals.
J Leukoc Biol
; 52(3): 315-22, 1992 Sep.
Article
em En
| MEDLINE
| ID: mdl-1381743
ABSTRACT
Trehalose dimycolate (TDM), a mycobacterial glycolipid, is a powerful macrophage-priming agent. However, its efficiency seems limited in the case of BALB/c mice. Peritoneal macrophages harvested from TDM-treated BALB/c mice did not control BCG growth in vitro as efficiently as similar macrophages from two other mouse strains, (B6 x D2)F1 and C57BL/6, which are respectively Bcgr and Bcgs. BALB/c macrophages elicited by TDM also exhibited a low capacity to produce hydrogen peroxide and, after activation by lipopolysaccharide (LPS), weak cytostatic activity against P815 mastocytoma cells. Finally, alkaline phosphodiesterase, a marker of resident and inflammatory macrophages, was still expressed at a high level in macrophages of BALB/c mice treated with TDM. Low responsiveness of BALB/c macrophages to stimuli was not observed with TDM only; activation for tumor cytotoxicity of thioglycolate-elicited macrophages from BALB/c mice required also higher doses of interferon-gamma, and LPS. L-Arginine-dependent production of nitric oxide was inducible in macrophages from BALB/c mice, but the conditions required for its induction were more stringent. Thus, the reduced antiproliferative effects of BALB/c macrophages may be due to uncomplete induction of NO synthase after suboptimal stimulation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trealose
/
Aminoácido Oxirredutases
/
Ativação de Macrófagos
/
Macrófagos
Limite:
Animals
Idioma:
En
Revista:
J Leukoc Biol
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
França