False-positive HIV antigens related to emergence of a 25-30 kD protein detected in organ recipients.
AIDS
; 6(9): 959-62, 1992 Sep.
Article
em En
| MEDLINE
| ID: mdl-1388908
ABSTRACT
OBJECTIVE:
The routine screening of organ donors for HIV-1 since 1985 has markedly reduced the risk of acquiring infection in organ recipients. However, commercial HIV-1 p24-antigen assays reveal false-positive reactivity in certain recipients. This observation will be discussed here.METHODS:
Post-transplantation sera collected sequentially from different organ recipients were tested for HIV antigen 79 samples were from 14 kidney recipients, 57 from seven bone-marrow allografts and 18 from two heart recipients. Neutralization assays to determine specificity were performed on reactive samples. Immunoblots prepared from sera containing high levels of antigens were tested by Western blot using polyclonal anti-HIV sera.RESULTS:
Abbott HIV-1-EIA kits detected non-neutralizable antigens in early post-transplantation sera from 12 kidney, five bone-marrow and two heart recipients. Using in-house immunoblots prepared from positive non-neutralizing antigen sera, a 25-30 kD protein was detected and shown to be the cause of the false HIV antigen cross-reactivity.CONCLUSION:
False-positive HIV antigens related to the emergence of a 25-30 kD protein in early post-transplantation sera are detectable in transplant recipients.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antígenos HIV
/
Transplante de Medula Óssea
/
Transplante de Coração
/
Transplante de Rim
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
AIDS
Assunto da revista:
SINDROME DA IMUNODEFICIENCIA ADQUIRIDA (AIDS)
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
França