Polypyrimidine tract-binding protein down-regulates fibroblast growth factor receptor 1 alpha-exon inclusion.
Cancer Res
; 63(19): 6154-7, 2003 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-14559795
ABSTRACT
Exclusion of the alpha-exon by alternative RNA splicing of the fibroblast growth factor receptor 1 (FGFR1) primary transcript leads to the production of FGFR1beta. Glial cell transformation is associated with a progressive increase in FGFR1beta expression that coincides with a dramatic increase in the expression of the splicing factor polypyrimidine tract-binding protein (PTB). Cell-specific overexpression of PTB increased alpha-exon skipping, and a reduction in PTB increased alpha-exon inclusion. Targeted disruption of PTB interaction with FGFR1 precursor RNA in vivo by an antisense oligonucleotide also increased alpha-exon inclusion. These results suggest that PTB plays a direct role in alpha-exon splicing.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de Fatores de Crescimento de Fibroblastos
/
Processamento Alternativo
/
Receptores Proteína Tirosina Quinases
/
Proteína de Ligação a Regiões Ricas em Polipirimidinas
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Estados Unidos