Rapid purification of RNA secondary structures.
Nucleic Acids Res
; 31(21): e135, 2003 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-14576335
ABSTRACT
A new method for rapid purification and structural analysis of oligoribonucleotides of 19 and 20 nt is applied to RNA hairpins SL3 and SL2, which are stable secondary structures present on the psi recognition element of HIV-1. This approach uses ion-pairing reversed-phase liquid chromatography (IP-RPLC) to achieve the separation of the stem-loop from the transcription mix. Evidence is presented that IP-RPLC is sensitive to the different conformers of these secondary structures. The purity of each stem-loop was confirmed by mass spectrometry and PAGE. IP-RPLC purification was found to be superior to PAGE in terms of time, safety and, most importantly, purity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Viral
/
Cromatografia Líquida
/
HIV-1
/
Sequências Reguladoras de Ácido Ribonucleico
/
Conformação de Ácido Nucleico
Idioma:
En
Revista:
Nucleic Acids Res
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Estados Unidos