Empirical analysis of transcriptional activity in the Arabidopsis genome.
Science
; 302(5646): 842-6, 2003 Oct 31.
Article
em En
| MEDLINE
| ID: mdl-14593172
ABSTRACT
Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. A dual experimental strategy was used to verify and correct the initial genome sequence annotation of the reference plant Arabidopsis. Sequencing full-length cDNAs and hybridizations using RNA populations from various tissues to a set of high-density oligonucleotide arrays spanning the entire genome allowed the accurate annotation of thousands of gene structures. We identified 5817 novel transcription units, including a substantial amount of antisense gene transcription, and 40 genes within the genetically defined centromeres. This approach resulted in completion of approximately 30% of the Arabidopsis ORFeome as a resource for global functional experimentation of the plant proteome.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
/
RNA Mensageiro
/
Arabidopsis
/
RNA de Plantas
/
Genoma de Planta
Idioma:
En
Revista:
Science
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Estados Unidos