Your browser doesn't support javascript.
loading
Ras gene mutations in patients with acute myeloid leukaemia and exposure to chemical agents.
Barletta, Emanuela; Gorini, Giuseppe; Vineis, Paolo; Miligi, Lucia; Davico, Laura; Mugnai, Gabriele; Ciolli, Stefania; Leoni, Franco; Bertini, Marilena; Matullo, Giuseppe; Costantini, Adele Seniori.
Afiliação
  • Barletta E; Department of Experimental Pathology and Oncology, University of Florence, Florence, Italy.
Carcinogenesis ; 25(5): 749-55, 2004 May.
Article em En | MEDLINE | ID: mdl-14688017
ABSTRACT
Mutations of the N- and K-ras genes occur in approximately 15-30% of acute myeloid leukaemia patients. The role of the oncogenic ras in leukaemogenesis remains unclear. Few studies have revealed that mutations in the ras oncogene family are more probably found in acute myeloid leukaemia patients with previous exposure to toxic agents. A case-case study was conducted in the areas of Florence and Turin, Italy, to investigate whether the presence of N- and K-ras mutations in acute myeloid leukaemia patients was related to a higher frequency of exposure to chemicals. During a 3-year period, 111 acute myeloid leukaemia patients were enrolled. All the patients were interviewed using a semi-structured questionnaire collecting data on residential history, occupation, personal habits and pathological history. The presence of N- and K-ras mutations was analysed by amplification and synthetic oligonucleotide probes and by the so-called polymerase chain reaction amplification for specific alleles technique. A total of 34 (30.6%) patients were found to harbour ras mutations in N-ras and/or K-ras. Fourteen patients (12.6%) had a single ras mutation and 20 patients (18%) had two ras mutations. A positive association between a priori at risk jobs and ras mutations was found, based on nine exposed cases; the odds ratio, adjusted by age, sex and previous X-ray and/or chemotherapy was 2.8 (95% confidence intervals 0.9-9.0). When considering only subjects with two ras mutations the odds ratio was 4.8 (95% confidence intervals 1.2-18.8). The odds ratio for a previous X-ray and/or chemotherapy was 16.2 (95% confidence intervals 1.8-755.9); when only subjects with two ras mutations were considered, the odds ratio was 26.1 (95% confidence intervals 2.5-1248.9). In conclusion, our data suggest that ras oncogene mutations might identify a group of leukaemia in people with previous X-ray/chemotherapy or with exposure to chemical agents in the work environment.
Assuntos
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Solventes / Leucemia Mieloide Aguda / Genes ras / Mutação Tipo de estudo: Prognostic_studies / Qualitative_research Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Carcinogenesis Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Itália
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Solventes / Leucemia Mieloide Aguda / Genes ras / Mutação Tipo de estudo: Prognostic_studies / Qualitative_research Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Carcinogenesis Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Itália