Use of expressed sequence tag analysis and cDNA microarrays of the filamentous fungus Aspergillus nidulans.
Fungal Genet Biol
; 41(2): 199-212, 2004 Feb.
Article
em En
| MEDLINE
| ID: mdl-14732266
ABSTRACT
The use of microarrays in the analysis of gene expression is becoming widespread for many organisms, including yeast. However, although the genomes of a number of filamentous fungi have been fully or partially sequenced, microarray analysis is still in its infancy in these organisms. Here, we describe the construction and validation of microarrays for the fungus Aspergillus nidulans using PCR products from a 4092 EST conidial germination library. An experiment was designed to validate these arrays by monitoring the expression profiles of known genes following the addition of 1% (w/v) glucose to wild-type A. nidulans cultures grown to mid-exponential phase in Vogel's minimal medium with ethanol as the sole carbon source. The profiles of genes showing statistically significant differential expression following the glucose up-shift are presented and an assessment of the quality and reproducibility of the A. nidulans arrays discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aspergillus nidulans
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Etiquetas de Sequências Expressas
/
Perfilação da Expressão Gênica
/
Glucose
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Fungal Genet Biol
Assunto da revista:
GENETICA
/
MICROBIOLOGIA
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Reino Unido