Kinetics of antibody binding at solid-liquid interfaces in flow.
J Immunol Methods
; 156(2): 223-30, 1992 Dec 08.
Article
em En
| MEDLINE
| ID: mdl-1474258
ABSTRACT
We have developed the theoretical framework for a displacement immunoassay conducted in flow under nonequilibrium conditions. Using a repetitive displacement technique, we determined the displacement rate and apparent dissociation rate constant at different flow rates. Our data suggest that the kinetics are best described by a first-order function. The displacement efficiency, the displacement rate, and therefore the apparent dissociation rate constant were calculated and demonstrated to be flow rate dependent. The theoretical framework developed in this study was successful in predicting the behavior of antigen displacement in flow.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoensaio
/
Reações Antígeno-Anticorpo
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1992
Tipo de documento:
Article