TbtABM, a multidrug efflux pump associated with tributyltin resistance in Pseudomonas stutzeri.
FEMS Microbiol Lett
; 232(1): 7-14, 2004 Mar 12.
Article
em En
| MEDLINE
| ID: mdl-15019728
ABSTRACT
Tributyltin (TBT) is a toxic agent used in marine antifouling paints. Among the bacterial flora of a polluted harbor, TBT-resistant strains of Pseudomonas stutzeri have been isolated. In the strain 5MP1 (TBT minimum inhibitory concentration (MIC) > or =1000 mg l(-1)), TBT resistance was found to be associated with the presence of the operon tbtABM, homologous to the resistance-nodulation-cell division (RND) efflux pump family, as demonstrated by cloning in Escherichia coli. TbtABM exhibited the greatest homology (60.9-84.9%) with the TtgDEF and SrpABC systems, both involved in aromatic compound tolerance in P. putida. TbtABM conferred multidrug resistance (MDR) including to n-hexane, nalidixic acid, chloramphenicol, and sulfamethoxazole (antibiotic MICsx4 for the E. coli host strain carrying the operon). By polymerase chain reaction amplification and hybridization experiments, the presence of tbtABM was detected in the TBT-sensitive P. stutzeri 3MP1 (TBT MIC 25 mg l(-1)). However, the latter strain did not seem to express TbtABM. This is the first description of a MDR efflux pump in P. stutzeri, and of a new kind of substrate, TBT, for the RND family of transporters.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Compostos de Trialquitina
/
Proteínas Associadas à Resistência a Múltiplos Medicamentos
/
Farmacorresistência Bacteriana Múltipla
/
Pseudomonas stutzeri
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
França