Fractalkine and its receptor, CX3CR1, upregulation in streptozotocin-induced diabetic kidneys.
Nephron Exp Nephrol
; 97(1): e17-25, 2004.
Article
em En
| MEDLINE
| ID: mdl-15153757
ABSTRACT
BACKGROUND:
Fractalkine is induced on activated endothelial cells and promotes strong adhesion of T cells and monocytes via its receptor CX3CR1. In kidney, fractalkine expression might be induced by high shear stress and play an important role in prolonged glomerular diseases. We examined whether fractalkine and CX3CR1 upregulation are found in streptozotocin-induced diabetic kidneys.METHODS:
Diabetic rats were randomized to receive an angiotensin-converting enzyme inhibitor (temocapril), aminoguanidine or no treatment. Reverse transcription-competitive polymerase chain reaction, Western blot analysis and immunohistochemistry were used.RESULTS:
At 4 weeks, fractalkine and CX3CR1 mRNA expression in diabetic kidneys increased compared with that in controls. Fractalkine staining in diabetic kidneys was clearly detected, along with glomerular capillary lumen and peritubular capillaries. A few CX3CR1 positive cell infiltration in diabetic glomeruli were found. Treatment with temocapril or aminoguanidine did not affect these changes. At 8 weeks, fractalkine and CX3CR1 mRNA expression in untreated diabetic kidneys markedly increased compared with that in controls. Membrane-anchored fractalkine protein expression in untreated diabetic rats also increased. The increased expression was suppressed by the treatment with temocapril and aminoguanidine. Increased CX3CR1-positive cell infiltration in diabetic glomeruli was also inhibited by both treatments. Some CX3CR1-positive cells were ED3 positive.CONCLUSIONS:
Fractalkine and CX3CR1 upregulation were demonstrated in an early stage of diabetic kidney. These upregulation, as well as urinary albumin excretion, were suppressed by treatments with temocapril and aminoguanidine for 8 weeks. These findings suggest that fractalkine expression and CX3CR1-positive cell infiltration in diabetic kidneys might play an important role for progression of diabetic nephropathy.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de HIV
/
Receptores de Citocinas
/
Quimiocinas CX3C
/
Diabetes Mellitus Experimental
/
Rim
/
Proteínas de Membrana
Limite:
Animals
Idioma:
En
Revista:
Nephron Exp Nephrol
Assunto da revista:
NEFROLOGIA
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Japão