Determination of CFTR chloride channel activity and pharmacology using radiotracer flux methods.
J Cyst Fibros
; 3 Suppl 2: 119-21, 2004 Aug.
Article
em En
| MEDLINE
| ID: mdl-15463942
ABSTRACT
Flux studies using either radioisotopes or ion-selective electrodes are a convenient method to assay the function of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel. Here, we described three different protocols to study the properties, regulation and pharmacology of the CFTR Cl- channel in populations of cells and artificial vesicles. These techniques are widely used to evaluate the function of wild-type and mutant CFTR prior to detailed analyses using the patch-clamp technique. Moreover, they have proved especially valuable in the search for new drugs to treat cystic fibrosis.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Membrana Celular
/
Técnicas Citológicas
/
Transporte de Íons
/
Regulador de Condutância Transmembrana em Fibrose Cística
Limite:
Humans
Idioma:
En
Revista:
J Cyst Fibros
Ano de publicação:
2004
Tipo de documento:
Article