Rapid detection of malaria infection in vivo by laser desorption mass spectrometry.
Am J Trop Med Hyg
; 71(5): 546-51, 2004 Nov.
Article
em En
| MEDLINE
| ID: mdl-15569781
ABSTRACT
Rapid diagnosis leading to effective treatment is essential to control escalating infectious diseases such as malaria. Malaria pigment (hemozoin) detection by laser desorption mass spectometry (LDMS) was recently shown to be a sensitive (<10 parasites/muL) technique for detecting Plasmodium falciparum parasites cultured in human blood. To examine the use of LDMS in a rapid new malaria screening assay, we followed the time course of P. yoelii infections in mice in parallel with light microscopy and a colorimetric hemozoin assay. Hemozoin was detected by LDMS in 0.3 muL of blood within two days of infection independently of the inoculating dose of 10(6), 10(4), or 10(2) parasite-infected erythrocytes. Microscopy and colorimetric hemozoin determinations lagged the LDMS detection of infections by 2-4 and 3-5 days, respectively, except at the highest inoculation dose. The LDMS detection of hemozoin is a potentially more rapid screen than light microscopy for detecting malaria infection in this mouse model at parasitemias <0.1%.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pigmentos Biológicos
/
Plasmodium yoelii
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Hemeproteínas
/
Malária
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Am J Trop Med Hyg
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Estados Unidos